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[Detection of mRNA in electronic microscopy: in situ hybridization of a biotin-labeled oligonucleotide prior to
1Laboratoire de Neurobiologie des Signaux Intercellulaires, CNRS URA 1488, Université P. et M. Curie, Paris, France.
Pathologie-Biologie
|February 1, 1993
Abstract:
A technique for mRNA detection by ultrastructural in situ hybridization is reported. Vibratome sections are hybridized with a biotinylated oligonucleotide probe which is detected using an enhanced avidin-peroxidase technique. Sections are then osmicated and embedded in epoxy resin. This rapid, sensitive technique achieved ultrastructural detection of vasopressin mRNA in magnocellular neurons of rat hypothalamus.