Related Experiment Video
Updated: Jul 28, 2026
05:58
The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF
Published on: November 18, 2009
Self-renewal of the long-term repopulating stem cell
G Brecher1, N Bookstein, W Redfearn
1Cell and Molecular Biology Division, Lawrence Berkeley Laboratory, Berkeley, CA 94720.
Summary
Single long-term repopulating cells (LTRCs) can fully restore blood cell systems in mice. The progeny of these LTRCs also possess repopulating ability, confirming stem cell self-renewal capacity.
Area of Science:
- Hematology
- Stem Cell Biology
- Immunology
Background:
- Self-renewal is a key characteristic of hematopoietic stem cells (HSCs).
- The self-renewal capacity of primitive long-term repopulating cells (LTRCs) has been debated.
- Understanding LTRC function is crucial for stem cell research and therapies.
Purpose of the Study:
- To investigate the self-renewal capacity of single long-term repopulating cells (LTRCs).
- To determine if LTRCs can regenerate the lymphohemopoietic system after transplantation.
- To explore competitive repopulation dynamics at low stem cell numbers.
Main Methods:
- Transplantation of single LTRCs into lethally irradiated mouse models.
- Serial transplantation experiments to assess progeny repopulating ability.
- Analysis of competitive marrow repopulation using low-dose cell transfusions.
Main Results:
- Single LTRCs successfully repopulated the lymphohemopoietic system in primary recipients.
- Progeny of single LTRCs in primary recipients retained LTRC activity for secondary recipients.
- Low-dose stem cell transfusions revealed successful competition by host cells, mirroring serial transplantation effects.
Conclusions:
- Demonstrates that single LTRCs possess robust self-renewal capabilities.
- Confirms the potential of LTRCs for complete lymphohemopoietic system regeneration.
- Highlights the importance of stem cell dose in competitive repopulation dynamics.
Related Concept Videos
01:19Multipotency of Hematopoietic Stem Cells
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
01:23Renewal of Intestinal Stem Cells
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...
01:12Renewal of Skin Epidermal Stem Cells
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
01:06Multipotency and Niche of Bulge Stem Cell
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
01:23Tissue Renewal without Stem Cells
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
However, failure of such a system...
01:14Maintenance of the ES Cell State
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...