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Published on: March 1, 2018
[Analysis of dextromethorphan and its metabolites in human urine by using gas chromatography-mass spectrometry]
1China Doping Control Center, National Research Institute of Sports Medicine, Beijing.
Abstract:
A GC-MS method for the analysis of dextromethorphan and its metabolites is described. The urine sample was hydrolyzed with HCl, extracted with diethyl ether and derivatized with MSTFA (N-methyl-N-trimethylsilyltrifluoroacetamide)-MBTFA (N-methyl-bistrifluoroacetamide). Dextromethorphan and its 3 metabolites were detected in urine samples within 2-60 h after administration of the drug. Their structures and the variation of their concentration in urine were determined. The detection limit of dextromethorphan is 10 ng.
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