Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interactions between mycoplasma pneumoniae and the first components of complement

W Bredt, B Wellek, H Brunner

    Infection and Immunity
    |January 1, 1977
    PubMed
    Summary

    Mycoplasma pneumoniae can be killed by guinea pig serum through an antibody-independent complement activation pathway. This involves the binding of the first component of complement (C1) to the mycoplasma surface, leading to cell death.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Evaluating the effect of spastin splice mutations by quantitative allele-specific expression assay.

    European journal of neurology·2010
    Same author

    Spectral composition of NREM sleep in healthy subjects with moderately increased daytime sleepiness.

    Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology·2003
    Same author

    Identification of cell surface determinants in Candida albicans reveals Tsa1p, a protein differentially localized in the cell.

    FEBS letters·2003
    Same author

    Child-Pugh versus MELD score in predicting survival in patients undergoing transjugular intrahepatic portosystemic shunt.

    Gut·2003
    Same author

    A screen in Saccharomyces cerevisiae identified CaMCM1, an essential gene in Candida albicans crucial for morphogenesis.

    Molecular microbiology·2003
    Same author

    EFG1 is a major regulator of cell wall dynamics in Candida albicans as revealed by DNA microarrays.

    Molecular microbiology·2002

    Area of Science:

    • Immunology
    • Microbiology
    • Complement System

    Background:

    • Mycoplasma pneumoniae is a significant human pathogen.
    • The complement system is a crucial part of innate immunity.
    • Antibody-dependent complement activation is a well-established mechanism.

    Purpose of the Study:

    • To investigate the mechanism of Mycoplasma pneumoniae killing by fresh guinea pig serum (GPS).
    • To determine if complement activation by M. pneumoniae is antibody-dependent.
    • To explore the interaction between M. pneumoniae and early complement components.

    Main Methods:

    • Incubation of M. pneumoniae with fresh guinea pig serum (GPS) and purified complement components.
    • Quantification of C1 binding to M. pneumoniae cells.

    Related Experiment Videos

  • Sequential addition of complement components (C1, C4, C2) to assess complement pathway activation.
  • Microscopic observation of cell morphology changes (rounding) and viability assays.

    • Main Results:

    • M. pneumoniae was killed by fresh GPS lacking detectable antibodies.
    • Significant binding of the first component of complement (C1) to M. pneumoniae was observed.
    • Sequential addition of C1, C4, and C2 led to cell rounding, indicating complement activation.
    • M. orale and M. fermentans showed reduced C1 binding and slower killing compared to M. pneumoniae.

    Conclusions:

    • M. pneumoniae activates the complement system via an antibody-independent mechanism.
    • The cell surface of M. pneumoniae directly interacts with C1, initiating complement cascade.
    • This interaction leads to complement-mediated killing of M. pneumoniae.