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Plasminogen activation activity of urokinase determined via a cascade enzymatic reaction system
W C Lee1, C Y Chuang, R M Chang
1Department of Chemical Engineering, Chung Yuan Christian University, Chung-Li, Taiwan.
Biochemistry and Molecular Biology International
|April 1, 1993
Abstract:
A method for determining the plasminogen activation rate by urokinase via a cascade enzymatic reaction system is presented. A procedure of parameter estimation has been proposed for the determination of the activity of urokinase and the kinetic constants. Urokinase from urine has been successfully assayed through use of a plasminogen concentration lower than its saturating level. The methodology presented in this work may be adopted for the analysis of other cascade enzymatic reaction systems.