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Related Experiment Videos

Coding end sequence can markedly affect the initiation of V(D)J recombination

R M Gerstein1, M R Lieber

  • 1Department of Pathology, Stanford University School of Medicine, California 94305-5324.

Genes & Development
|July 1, 1993
PubMed
Summary

The nucleotide sequence at coding ends significantly impacts V(D)J recombination efficiency, affecting antigen receptor diversity. This suggests signal-binding proteins interact differently with varied coding end sequences.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • V(D)J recombination is crucial for generating diverse antigen receptors.
  • Deviations in signal sequences impact recombination efficiency.
  • Coding end sequences were previously assumed to be recombination-neutral.

Purpose of the Study:

  • To systematically investigate the impact of coding end sequences on V(D)J recombination efficiency.
  • To determine if coding end sequence variations influence recombination rates.

Main Methods:

  • Utilized extrachromosomal V(D)J recombination substrates.
  • Constructed substrates with identical consensus recombination signal sequences.
  • Varied only the nucleotide sequences at the coding ends for comparison.

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Main Results:

  • Coding end sequences can influence V(D)J recombination efficiency by over 250-fold.
  • Variable initiation of recombination appears to be the primary mechanism for this effect.
  • Signal-binding proteins exhibit differential activity based on coding end sequences.

Conclusions:

  • Coding end sequence is a major factor influencing V(D)J recombination efficiency.
  • This finding has mechanistic implications for V(D)J recombination pathways.
  • Coding end sequence variability is critical for shaping the antigen receptor repertoire.