High performance liquid chromatographic analysis of supplemental vitamin E in feed

Supplemental vitamin E is extracted from feed in one step with methanol and analyzed by reverse phase partition chromatography in less than 10 min, using isocratic elution with either methanol or water-methanol as the mobile phase. Detection response (as measured by peak area) was linear between 0.5 and 3.0 mug, and the coefficients of variation for retention time and peak height on replicate analyses of the standard sample were 0.8 and 2.3%, respectively.