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Experimentally induced dentoalveolar ankylosis in rats
E Hellsing1, I Alatli-Kut, L Hammarström
1Department of Orthodontics, School of Dentistry, Karolinska Institute, Huddinge, Sweden.
International Endodontic Journal
|March 1, 1993
Summary
Dentoalveolar ankylosis, a complication of tooth reimplantation, causes root resorption and exfoliation. This study developed a rat model to investigate factors influencing ankylosis development and progression.
Area of Science:
- Oral and Maxillofacial Surgery
- Dental Traumatology
- Regenerative Dentistry
Background:
- Dentoalveolar ankylosis is a significant complication following tooth reimplantation, characterized by the fusion of the tooth root to the alveolar bone.
- This condition leads to progressive root resorption and can cause infra-occlusion and impaired alveolar development in younger patients.
Purpose of the Study:
- To establish a reproducible rat model for studying the development of dentoalveolar ankylosis.
- To investigate the influence of systemic and local factors on ankylosis progression and the functional lifespan of reimplanted teeth.
Main Methods:
- Extraction of maxillary first molars in adult rats.
- Devitalization or removal of periodontal ligament (PDL) cells using Dakin's solution.
- Replantation of teeth and maintenance on a liquid diet for up to 3 weeks post-replantation.
Main Results:
- Dentoalveolar ankylosis was reliably induced within 2 weeks post-replantation.
- Exfoliation typically occurred around 6 weeks.
- Histological analysis revealed PDL replacement by bone, varying degrees of root resorption, and common findings of bone-like tissue in the root canal.
Conclusions:
- The developed rat model effectively replicates key features of dentoalveolar ankylosis.
- This model provides a platform for further research into therapeutic interventions for ankylosis and root resorption.