Related Experiment Video

Updated: Aug 13, 2026

Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle
11:57

Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle

Published on: March 22, 2013

Striated myofibrils in anti-myosin stained, isolated chicken gizzard smooth muscle cells

R M Bagby, F A Pepe

    Histochemistry
    |December 1, 1978
    PubMed

    Highly purified chicken gizzard myosin was used to induce antibody production in rabbits. The IgG fraction was separated from the antisera and coupled to fluorescein isothiocyanate (FITC). Specific antibody (AGM) was isolated from the IgG fraction by affinity purification. Comparisons of the specificity of IgG and AGM for chicken smooth muscle myosin revealed a much greater specificity by AGM. Staining with IgG led to an apparent cross-reactivity with guinea pig smooth muscles which was not seen with AGM staining. Therefore, staining of cells for localization of myosin was performed with AGM. Isolated cells were obtained from chicken gizzards either by collagenase digestion or by agitation of glycerinated pieces. Stained cells and cell fragments revealed the presence of myofibrils as structural units with diameters of about 1.0 micrometer. Stained myofibrils occasionally displayed regular banding patterns with a repeating period of about 1.5 +/- 0.2 micrometer. The presence of banded myofibrils in non-cultured cells shows that the organization of the contractile material is similar to that previously reported for cultured cells by Gröschel-Stewart.

    More Related Videos

    Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles07:37

    Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles

    Published on: July 20, 2015

    Immunolabelling Myofiber Degeneration in Muscle Biopsies06:37

    Immunolabelling Myofiber Degeneration in Muscle Biopsies

    Published on: December 5, 2019

    Related Concept Videos

    Skeletal Muscle Anatomy00:55

    Skeletal Muscle Anatomy

    Skeletal muscle is the most abundant type of muscle in the body. Tendons are the connective tissue that attaches skeletal muscle to bones. Skeletal muscles pull on tendons, which in turn pull on bones to carry out voluntary movements.
    Classification of Skeletal Muscle Fibers01:48

    Classification of Skeletal Muscle Fibers

    Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions.
    Slow-Twitch Muscle Fibers
    Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
    Overview of Myosin Structure and Function01:15

    Overview of Myosin Structure and Function

    Myosins are a family of molecular motor proteins, first identified in the skeletal muscles, where they are responsible for muscle contraction. Along with their role in muscle contraction, these proteins also play a role in the intracellular transport of molecules and vesicles. There are twenty-four classes of myosins based on their domain sequence and organization. Of the twenty-four, six classes (Myosin I, Myosin II, Myosin V, Myosin VI, Myosin VII, and Myosin X)  have been well characterized.
    Actin and Myosin in Muscle Contraction01:16

    Actin and Myosin in Muscle Contraction

    Actin and myosin are contractile proteins that form the sarcomere found in skeletal muscle tissues for regulating muscle contraction. Actin, a globular contractile protein, interacts with myosin for muscle contraction. The skeletal tissue appears striped or striated under a microscope due to the repeated arrangement of contractile proteins actin and myosin along the length of myofibrils. Dark A bands and light I bands repeat along myofibrils, and the alignment of myofibrils in the cell causes...
    Microscopic Anatomy of Skeletal Muscles01:13

    Microscopic Anatomy of Skeletal Muscles

    Skeletal muscle cells, also called muscle fibers, are distinctly elongated, multi-nucleated, slender biological units. They are packed with specialized structures designed to facilitate their primary function, which is contraction.
    The muscle sarcolemma is a plasma membrane enclosing each muscle cell that conducts electrical signals called action potentials. The sarcolemma extends into the cell to form T-tubules, ensuring the neural impulses are uniformly distributed across the entire muscle...
    Smooth Muscle Contraction01:25

    Smooth Muscle Contraction

    Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
    The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...