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Intermediate filaments in nervous tissues

R K Liem, S H Yen, G D Salomon

    The Journal of Cell Biology
    |December 1, 1978
    PubMed
    Summary
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    Researchers isolated intermediate filaments from spinal nerve roots and brain, identifying distinct protein compositions for neurofilaments and glial filaments. This research clarifies the molecular differences between these crucial cellular structures.

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biochemistry

    Background:

    • Intermediate filaments are crucial cytoskeletal components in neurons and glia.
    • Neurofilaments and glial filaments are distinct types of intermediate filaments with different functions.
    • Understanding their molecular composition is key to understanding neuronal and glial biology.

    Purpose of the Study:

    • To isolate and characterize intermediate filaments from rabbit spinal nerve roots.
    • To compare the protein composition of neurofilaments and glial filaments.
    • To investigate the differential solubility of neurofilaments and glial filaments.

    Main Methods:

    • Axonal flotation method modified to preserve filament integrity.
    • Gel electrophoresis to analyze polypeptide composition.

    Related Experiment Videos

  • Immunofluorescence using antibodies against specific polypeptides.

    • Main Results:

    • Spinal nerve root filaments showed polypeptides at 200,000, 145,000, 68,000, and 60,000 daltons.
    • Brain preparations revealed polypeptides at 200,000, 145,000, 68,000, and 51,000 daltons.
    • The 51,000-dalton polypeptide was identified as glial, while the triplet (200,000, 145,000, 68,000 daltons) constitutes neurofilaments.
    • Neurofilaments are soluble in low ionic strength, while glial filaments are not.

    Conclusions:

    • Neurofilaments and glial filaments are composed of distinct polypeptides.
    • Differential solubility characteristics further distinguish neurofilaments from glial filaments.
    • This study provides a molecular basis for differentiating neuronal and glial intermediate filaments.