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Updated: Aug 4, 2026

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
Published on: April 17, 2015
High throughput DNA sequencing using an automated electrophoresis analysis system and a novel sequence assembly
S Smith1, W Welch, A Jakimcius
1Millipore Corporation, Burlington, MA 01803.
Abstract:
The combination of high-resolution scanning, image-databasing technology and sequence assembly software makes it possible to assemble contiguous overlapping sequences of DNA in a fraction of the time required by manual methods. This paper describes and evaluates an assembly program that rapidly generates contigs from scanned images and provides a unique ability to verify disagreements between overlapping strands or fragments of complementary DNA sequences on-screen, further increasing sequencing throughput.
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