Related Experiment Videos
Cloning and sequence analysis of an esterase gene from Pseudomonas sp. KWI-56
Y Shimada1, T Nagao, A Sugihara
1Osaka Municipal Technical Research Institute, Japan.
Biochimica Et Biophysica Acta
|July 18, 1993
Abstract:
The gene encoding an esterase from Pseudomonas sp. KWI-56 was cloned and sequenced. The nucleotide sequence contained an open reading frame encoding a polypeptide comprising 262 amino acids, whose molecular weight agreed well with the value obtained by SDS-PAGE. Comparison of the amino acid sequence with those of the other homologous enzymes suggested that Ser-92 and His-24l might be included in the catalytic triad.