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[Microbiological control in parenteral nutrition mixtures. Indices of contamination]
F Ginés Rubió1, F Puigventós Latorre, A Escrivá Torralva
1Servicio de Farmacia, Hospital Son Dureta, Palma de Mallorca, Spain.
Abstract:
Potential contamination of nutritional mixtures during preparation in the Pharmacy Services has led to the need for microbiological controls. In our study, we set out the techniques used in the Son Dureta Hospital between 1986 and 1991. During those six years 11,023 nutritional mixtures were prepared, and 1,714 bacteriological controls were carried out, representing 15.5% of the total. Between January 1986 and June 1989, the simple culture technique was used (culture of 10 ml of the prepared mixture). The contamination rate was 1.27%. Between July 1989 and December 1991, the double sample technique was applied, involving a culture of 10 ml of the mixture, keeping a further 10 ml for subsequent verification culture. With this technique, the contamination rate was fixed at 0.34%. The advantages and drawbacks of the techniques used are described, along with the problem of initial false positives, and a review is made of the 39 germs isolated during the period of study.
Insights
Implementing a double sample technique for microbiological controls in nutritional mixtures significantly reduced contamination rates from 1.27% to 0.34%. This study highlights improved safety in pharmacy services preparation.
Area of Science:
- Microbiology
- Pharmaceutical Sciences
- Clinical Pharmacy
Context:
- Potential for microbial contamination during the preparation of nutritional mixtures in hospital pharmacy services necessitates robust microbiological controls.
- This study evaluates microbiological control techniques implemented at Son Dureta Hospital between 1986 and 1991.
Purpose:
- To assess and compare the effectiveness of different microbiological control techniques for prepared nutritional mixtures.
- To determine the impact of technique modification on the rate of microbial contamination.
Summary:
- Over six years, 11,023 nutritional mixtures were prepared, with 1,714 bacteriological controls performed (15.5%).
- The simple culture technique (10 ml sample) yielded a 1.27% contamination rate (Jan 1986 - Jun 1989).
- The double sample technique (10 ml initial culture, 10 ml verification culture) reduced contamination to 0.34% (Jul 1989 - Dec 1991).
Impact:
- The adoption of the double sample technique significantly decreased microbial contamination rates in prepared nutritional mixtures.
- Findings provide valuable insights into optimizing microbiological surveillance in pharmaceutical preparations.
- Discussion includes the advantages and disadvantages of each method and an analysis of 39 isolated microorganisms.