Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Allergens from Dermatophagoides mites with chymotryptic activity

H Yasueda1, H Mita, K Akiyama

  • 1Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital, Japan.

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
|May 1, 1993
PubMed
Summary

New mite allergens, DP5 and DF5, were identified and characterized as serine proteases. While these allergens showed proteolytic activity, they were recognized by IgE in fewer allergic patients compared to established mite allergens.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prognostic value of power doppler ultrasonography for equine superficial digital flexor tendon injury in thoroughbred racehorses.

Veterinary journal (London, England : 1997)·2024
Same author

Evaluation of large airway specimens obtained by transbronchial lung cryobiopsy in diffuse parenchymal lung diseases.

BMC pulmonary medicine·2022
Same author

Terahertz-field-induced polar charge order in electronic-type dielectrics.

Nature communications·2021
Same author

Autoimmune pulmonary alveolar proteinosis developed during immunosuppressive treatment in polymyositis with interstitial lung disease: a case report.

BMC pulmonary medicine·2020
Same author

Doublon-holon pairing mechanism via exchange interaction in two-dimensional cuprate Mott insulators.

Science advances·2019
Same author

Ultrafast polarization control by terahertz fields via π-electron wavefunction changes in hydrogen-bonded molecular ferroelectrics.

Scientific reports·2018

Area of Science:

  • Immunology
  • Allergology
  • Biochemistry

Background:

  • Dermatophagoides pteronyssinus and Dermatophagoides farinae are common house dust mites.
  • Mite allergens are a significant cause of allergic respiratory diseases worldwide.
  • Characterization of novel mite allergens is crucial for understanding allergic sensitization.

Purpose of the Study:

  • To isolate and characterize novel allergens from Dermatophagoides pteronyssinus and Dermatophagoides farinae.
  • To investigate the enzymatic activity and classification of these new allergens.
  • To assess the IgE reactivity of these novel allergens in mite-allergic patients.

Main Methods:

  • Isolation of allergens from whole mite culture.
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Sephadex G-75 gel filtration for molecular weight determination.

Related Experiment Videos

  • Proteolytic activity assays, substrate specificity, and protease inhibitor susceptibility studies.
  • Radio-allergosorbent test (RAST) to detect specific IgE antibodies in patient sera.

    • Main Results:

    • Two new allergens, provisionally named DP5 and DF5, were isolated.
    • DP5 and DF5 exhibited apparent molecular weights of 25,000 (reducing SDS-PAGE) and 27,000 (Sephadex G-75).
    • Both DP5 and DF5, along with Der f III, demonstrated proteolytic activity consistent with chymotrypsin-like serine proteases.
    • Specific IgE antibodies to DP5 and/or DF5 were detected in only 41% of mite-allergic patients, contrasting with high reactivity to Der p I/Der f I (90%) and Der p II/Der f II (93%).

    Conclusions:

    • DP5 and DF5 are novel chymotrypsin-like serine protease allergens from Dermatophagoides species.
    • These newly identified allergens show lower IgE recognition among mite-allergic patients compared to established major allergens Der p I/Der f I and Der p II/Der f II.
    • Further research is needed to elucidate the role of DP5 and DF5 in mite allergy.