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Annexin 1 is present in different molecular forms in rat cerebral cortex
1Unité CNRS UA 1112, Neurobiologie Physico-Chimique, Paris, France.
FEBS Letters
|July 19, 1993
Summary
Annexin 1, previously identified as a 37 kDa monomer in pig brain, is found as a 74 kDa dimer in rat brain synaptic plasma membranes. This suggests enzymatic dimerization occurs when annexin 1 associates with the membrane.
Area of Science:
- Neuroscience
- Molecular Biology
- Cell Biology
Background:
- Annexin 1 was previously purified from pig cerebral cortex as a 37 kDa monomer.
- The localization and molecular form of annexin 1 in the rat brain were not fully understood.
Purpose of the Study:
- To investigate the subcellular localization of annexin 1 in the rat cerebral cortex.
- To determine the molecular form of annexin 1 in synaptic fractions.
Main Methods:
- Subcellular fractionation of rat cerebral cortex.
- Immunodetection using a specific antibody against annexin 1.
- SDS-PAGE analysis to determine molecular weight.
Main Results:
- Annexin 1 was localized to the synaptic plasma membrane fraction, distinct from synaptic vesicle markers like synaptophysin.
- Annexin 1 appeared as a 74 kDa polypeptide in the synaptic plasma membrane fraction.
- The 74 kDa form was also extracted from purified synaptic plasma membranes.
Conclusions:
- Annexin 1 is present in the synaptic plasma membrane of rat cerebral cortex.
- A 74 kDa molecular form of annexin 1 suggests enzymatic dimerization upon membrane association.