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[Stabilization of ascorbic acid aqueous solution by protamine]

S Yamauchi1, N Nimura, T Kinoshita

  • 1Central Research Laboratory, SS Pharmaceutical Co. Ltd., Chiba, Japan.

Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|May 1, 1993
PubMed
Summary

Protamine effectively stabilizes ascorbic acid (AsA) in aqueous solutions, significantly reducing degradation. This protective effect is particularly notable in the presence of copper ions and across a range of pH levels.

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Area of Science:

  • Biochemistry
  • Chemical Kinetics

Context:

  • Ascorbic acid (AsA) is a vital nutrient prone to degradation in aqueous environments.
  • Metal ions, such as copper (II), can catalyze AsA oxidation.
  • Understanding AsA stability is crucial for pharmaceutical and food science applications.

Purpose:

  • To investigate the stabilizing effect of protamine on ascorbic acid in aqueous solutions.
  • To evaluate protamine's efficacy in mitigating AsA degradation catalyzed by copper ions.
  • To determine the influence of pH on protamine's protective action towards AsA.

Summary:

  • Protamine significantly enhances ascorbic acid (AsA) stability in a 0.05 M phosphate buffer at pH 7 and 30°C.
  • In the presence of 60 µmol/l protamine, AsA concentration remained at 91% after 90 minutes, compared to a 57% decrease without protamine.

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  • Protamine also protected AsA from copper (II) ion-induced degradation, maintaining 52% AsA after 90 minutes compared to 5% degradation without protamine.
  • The stabilizing effect of protamine on AsA was observed above pH 5, effectively controlling degradation at higher pH values, especially in the presence of copper ions.

    • Impact:

    • Protamine demonstrates potential as a stabilizing agent for ascorbic acid formulations.
    • This finding could lead to improved shelf-life and efficacy of products containing ascorbic acid.
    • The study highlights protamine's role in preventing oxidative degradation of sensitive molecules.