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Introducing a biosensor based technology for real-time biospecific interaction analysis

U Jönsson1, L Fägerstam, S Löfas

  • 1Pharmacia Biosensor AB, Uppsala, Sweden.

Annales De Biologie Clinique
|January 1, 1993
PubMed
Summary
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This study introduces a versatile biosensor system for real-time analysis of biomolecular interactions using surface plasmon resonance. It enables label-free detection and characterization of molecular binding events with high sensitivity and broad applicability.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biophysics

Background:

  • Biospecific interaction analysis is crucial for understanding biological processes.
  • Existing methods often require molecular labels or extensive purification.
  • A need exists for sensitive, versatile, and label-free analytical systems.

Purpose of the Study:

  • To describe a novel system for real-time biospecific interaction analysis.
  • To demonstrate the system's versatility and applicability across various binding studies.
  • To highlight the advantages of surface plasmon resonance (SPR) biosensor technology.

Main Methods:

  • Utilized a biosensor chip with a thin gold film and hydrogel matrix for a stable biospecific interface.
  • Covalently attached one interaction partner to the hydrogel, flowing the other over the chip.

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  • Monitored interactions in real-time by detecting changes in mass concentration via surface plasmon resonance.

    • Main Results:

    • Achieved real-time detection of biospecific interactions with sensitivity down to 10 pg/mm2.
    • Demonstrated label-free analysis, often avoiding the need for molecular labels or purification.
    • Showcased versatility for concentration determination, kinetic measurements, and multi-site binding studies.
    • Documented protein concentration measurements down to 20 ng/ml and association constants from 10(7) M-1 to 4 x 10(11) M-1.

    Conclusions:

    • The developed system offers unparalleled versatility and general applicability for biospecific interaction analysis.
    • Surface plasmon resonance biosensor technology provides a powerful label-free approach for studying molecular interactions.
    • The system supports a wide range of applications, from basic research to diagnostics.