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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
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Ontogeny of hematopoietic stem cell development: reciprocal expression of CD33 and a novel molecule by maturing

C Brashem-Stein1, D A Flowers, F O Smith

  • 1Programs in Pediatric Oncology, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.

Blood
|August 1, 1993
PubMed

We have identified a molecule expressed by human marrow granulocyte/monocyte colony-forming cells (CFU-GM), erythroid colony-forming cells (CFU-E), and erythroid burst-forming units (BFU-E), but not their precursors detectable in long-term bone marrow culture. This antigen, detected by flow microfluorimetry using monoclonal antibody 7B9, is coexpressed with CD33 on many CD34+ CFCs, although only the 7B9 antigen was detected on a portion of BFU-E and CFU-E, whereas only CD33 was found on a portion of CFU-GM. Antibody 7B9 appears to be useful for isolating subsets of progenitors based on their common or selective expression of 7B9 antigen and CD33.

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