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Factors affecting sperm yield during swim-up

K Purvis1, I Egdetveit

  • 1Andrology Laboratory, National Hospital, Oslo, Norway.

Journal of Assisted Reproduction and Genetics
|February 1, 1993
PubMed
Summary
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Optimizing sperm swim-up involves increasing incubation time, temperature, and tube angle for better sperm migration. While sperm count didn't affect migration, motility-enhancing drugs offered only temporary benefits for sperm yield.

Area of Science:

  • Reproductive biology
  • Spermatozoa research
  • Assisted reproductive technologies

Background:

  • Sperm preparation is crucial for assisted reproductive technologies.
  • The swim-up technique is a common method for selecting motile sperm.
  • Understanding factors influencing sperm migration is essential for optimizing protocols.

Purpose of the Study:

  • To investigate the impact of time, temperature, and physical parameters on sperm migration during the swim-up procedure.
  • To evaluate the effect of sperm concentration and motility-enhancing drugs on sperm yield.

Main Methods:

  • Sperm migration (swim-up) was assessed under varying incubation times and temperatures.
  • The surface area for sperm migration was manipulated by altering tube inclination angles.

Related Experiment Videos

  • The effect of sperm count and a motility-enhancing drug (2-deoxyadenosine) on migration was studied.

    • Main Results:

    • Sperm migration increased with incubation time, temperature, and tube angle.
    • Sperm count did not significantly influence the proportion of migrating sperm.
    • Motility-enhancing drugs provided an initial boost but did not surpass control yields after prolonged incubation.

    Conclusions:

    • Sperm yield from normozoospermic and borderline asthenoteratozoospermic men was approximately 40% and 15% of motile cells, respectively.
    • Sperm in the swim-up fraction consistently showed improved motility, vitality, and morphology compared to the original ejaculate.
    • Improvements were more pronounced in normozoospermic individuals, with tail abnormalities showing greater reduction than head abnormalities.