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DNA contained by two densonucleosis viruses

D C Kelly, A H Barwise, I O Walker

    Journal of Virology
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

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    Densonucleosis viruses 1 and 2 contain single-stranded DNA within particles. This DNA can be extracted as double-stranded DNA, revealing high homology between the two viruses.

    Area of Science:

    • Virology
    • Molecular Biology
    • Genetics

    Background:

    • Densonucleosis viruses (DNVs) are small, non-enveloped viruses known to infect insects.
    • Understanding the structure and properties of viral DNA is crucial for comprehending viral replication and evolution.

    Purpose of the Study:

    • To analyze the DNA within Densonucleosis viruses 1 and 2 (DNV-1 and DNV-2) particles.
    • To determine the properties of DNA extracted from these viral particles.

    Main Methods:

    • Spectroscopic analysis (formaldehyde-induced spectral changes)
    • Thermal denaturation (melting profiles)
    • Circular dichroism spectroscopy
    • Molecular weight determination (ultracentrifugation, electron microscopy)
    • Reassociation kinetics

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    Main Results:

    • DNV-1 and DNV-2 DNA exists as single-stranded molecules within viral particles, exhibiting limited secondary structure.
    • The single-stranded DNA has an apparent molecular weight of 1.9-2.2 x 10^6 Da.
    • Double-stranded DNA (dsDNA) can be extracted under high salt and elevated temperatures.
    • The extracted linear dsDNA has a molecular weight of approximately 3.9-4.1 x 10^6 Da.
    • Reassociation kinetics indicate an equivalent genome size for the dsDNA.
    • Approximately 87% of the DNA sequences are homologous between DNV-1 and DNV-2.

    Conclusions:

    • Densonucleosis viruses 1 and 2 possess single-stranded DNA genomes that can be converted to double-stranded form.
    • The high degree of DNA homology suggests a close evolutionary relationship between DNV-1 and DNV-2.