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Updated: Aug 10, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
[Differentiation of staphylococci from sheep and goat milk samples]
1II. Medizinischen Universitätsklinik für Klauentiere der Veterinärmedizinischen Universität Wien.
Abstract:
A total of 447 micrococcaceae strains isolated from 88 ewe and 359 goat milk samples from cases of chronic mastitis were differentiated by means of the ATB 32 STAPH-test. Of these strains 389 (= 87%) could be identified. Fourteen strains were sensitive in the bacitracin-resistance-test and therefore classified as Micrococcus spp. In ewe milk following Staphylococcus spp. were found: S. epidermidis, S. aureus, S. lentus, S. xylosus, S. warneri, S. equorum, S. haemolyticus, S. simulans, S. hominis and S. saprophyticus. Staphylococcus spp. identified in goat milk samples were: S. epidermidis, S. aureus, S. caprae, S. lentus, S. simulans, S. capitis, S. lugdunensis, S. xylosus, S. chromogenes, S. hominis, S. arlettae, S. warneri, S. sciuri, and S. saprophyticus. Highest cell counts in the milk of both animals species, and the highest incidence of clinical udder alterations were caused by S. aureus. Increases in milk cell counts as well as pathological udder findings were observed in coagulase-negative staphylococcal infections for novobiocin-sensitive Staphylococcus spp. (S. epidermidis, S. warneri, S. simulans, S. lugdunensis, and S. chromogenes) and several S. lentus strains.
Insights
Staphylococcus aureus and coagulase-negative staphylococci, particularly S. epidermidis, are key causes of chronic mastitis in ewes and goats. These bacteria significantly increase milk somatic cell counts and lead to pathological udder changes.
Area of Science:
- Veterinary Microbiology
- Dairy Science
- Bacterial Pathogenesis
Context:
- Chronic mastitis is a significant concern in small ruminant dairy production, impacting both ewe and goat health and milk quality.
- Micrococcaceae, particularly Staphylococcus species, are frequently implicated as causative agents of mastitis in dairy animals.
Purpose:
- To identify and differentiate Micrococcaceae strains, focusing on Staphylococcus species, isolated from chronic mastitis cases in ewe and goat milk.
- To correlate specific Staphylococcus species with the severity of mastitis, including milk somatic cell counts and clinical udder alterations.
Summary:
- A total of 447 Micrococcaceae strains were isolated from 447 ewe and goat milk samples. Of these, 389 (87%) were identified using the ATB 32 STAPH-test.
- Staphylococcus aureus was identified as the primary cause of highest milk cell counts and clinical udder alterations in both species.
- Coagulase-negative staphylococci, including S. epidermidis, S. warneri, S. simulans, S. lugdunensis, and S. chromogenes, were associated with increased milk cell counts and pathological findings.
Impact:
- This study provides crucial insights into the specific Staphylococcus species involved in chronic mastitis in small ruminants.
- Understanding the pathogenic potential of different staphylococci can inform targeted prevention and treatment strategies for mastitis in sheep and goats.
- The findings contribute to improving dairy animal health management and milk production efficiency by highlighting key bacterial culprits and their effects.
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