[Study on determination of gentamycin by enhanced chemiluminescent immunoassay]

Gentamycin was determined by solid antigen competition inhibition method of chemiluminescet immunoassay, in which luminol-(p-hydroxydiphenyl)-H2O2 was used a the chemiluminescence system and p-hydroxydiphenyl as the chemiluminescent enhancer. Solid antigen was synthesized by a method in which gentamycin was immobilized covalently on microcrystalline cellulose previously activated by 1,1'-carbonyldiimidazole. The rabbit anti-gentamycin IgG and the HRP-donkey anti-rabbit IgG were used as the first antibody and labelled second antibody, respectively. The linear range of the gentamycin (with blank serum) was 0.03-1 micrograms/tube and the correlation coefficient of the log-log regression equation [r[ was 0.981 +/- 0.016 with a limit of detection 3.3-11.4 ng/tube and the recovery of gentamycin 88.2 +/- 4.5%.