Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ascorbic acid recycling in human neutrophils

P W Washko1, Y Wang, M Levine

  • 1Section of Cell Biology and Biochemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.

The Journal of Biological Chemistry
|July 25, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Different core promoters possess distinct regulatory activities in the Drosophila embryo.

Genes & development·1998
Same author

Rush to judgment? Child protective services and allegations of sexual abuse.

The American journal of orthopsychiatry·1998
Same author

In vivo cimetidine inhibits hepatic CYP2C6 and CYP2C11 but not CYP1A1 in adult male rats.

The Journal of pharmacology and experimental therapeutics·1998
Same author

Pulmonary toxicity of high-dose chemotherapy for breast cancer: a non-invasive approach to diagnosis and treatment.

Bone marrow transplantation·1998
Same author

Ascorbate recycling in human neutrophils: induction by bacteria.

Proceedings of the National Academy of Sciences of the United States of America·1998
Same author

The occurrence of subsequent malignancy in patients presenting with deep vein thrombosis: results from a historical cohort study.

Thrombosis and haemostasis·1998
Same journal

Wanted and unwanted modifications of mRNA, and their effect on gene expression and signaling.

The Journal of biological chemistry·2026
Same journal

TGF-β2 drives lipid droplet accumulation in chondrocytes through the TβRI/p-Smad3/Fabp5 axis.

The Journal of biological chemistry·2026
Same journal

Macrophage-specific targeting of histone demethylases with small-molecule inhibitors suppresses inflammatory response in vivo.

The Journal of biological chemistry·2026
Same journal

Substrate and target selectivity of 4'-fluoroadenosine against viral and host polymerases.

The Journal of biological chemistry·2026
Same journal

Correction: Characterization of Mast2 kinase defines structural features, regulation, and substrates.

The Journal of biological chemistry·2026
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
See all related articles

Activated human neutrophils accumulate 10x more vitamin C (ascorbic acid) by utilizing and recycling its oxidized form, dehydroascorbic acid, under physiological conditions.

Area of Science:

  • Biochemistry
  • Immunology
  • Cell Biology

Background:

  • Neutrophils play a crucial role in the immune response.
  • Ascorbic acid (vitamin C) is essential for various cellular functions.
  • Understanding vitamin C transport in immune cells is important for immune health.

Purpose of the Study:

  • To investigate the mechanism and extent of ascorbic acid accumulation in activated human neutrophils.
  • To determine if neutrophils can utilize and recycle oxidized vitamin C.

Main Methods:

  • Measuring intracellular ascorbic acid concentrations in activated human neutrophils.
  • Analyzing the transport of dehydroascorbic acid across the neutrophil membrane.
  • Observing the intracellular reduction of dehydroascorbic acid to ascorbic acid.

Related Experiment Videos

Main Results:

  • Activated human neutrophils accumulate ascorbic acid up to 10-fold higher than normal neutrophils.
  • Internal concentrations can reach 14 mM under physiological external vitamin C levels.
  • Neutrophils efficiently transport dehydroascorbic acid and reduce it intracellularly.

Conclusions:

  • Activated human neutrophils exhibit significantly enhanced ascorbic acid accumulation.
  • Neutrophils possess a mechanism to utilize and recycle oxidized vitamin C (dehydroascorbic acid).
  • This process allows neutrophils to maintain high intracellular vitamin C levels for optimal function.