Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tissue generation with growth factors

R K Khouri1, B Koudsi, E G Deune

  • 1Department of Surgery, Washington University School of Medicine, St. Louis, Mo.

Surgery
|August 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Different methods in predicting mortality of pediatric intensive care units sepsis in Southwest China].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2024
Same author

[Determination of 1-methoxy-2-propanol in urine by headspace solid-phase microextraction coupled with gas chromatography].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2020
Same author

Dietary threonine prevented stress-related mucosal diseases in rats.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2019
Same author

Genotypes, phenotypes and whole genome sequence: Approaches from the My Life Our Future haemophilia project.

Haemophilia : the official journal of the World Federation of Hemophilia·2018
Same author

Past, present and future of haemophilia gene therapy: From vectors and transgenes to known and unknown outcomes.

Haemophilia : the official journal of the World Federation of Hemophilia·2018
Same author

Core outcome set for gene therapy in haemophilia: Results of the coreHEM multistakeholder project.

Haemophilia : the official journal of the World Federation of Hemophilia·2018
Same journal

Familiar but unprepared: Artificial intelligence training needs in graduate medical education.

Surgery·2026
Same journal

One-year health care expenditures and patient out-of-pocket spending after open versus minimally invasive hepatopancreatobiliary surgery.

Surgery·2026
Same journal

Shock index, hypotension, and blood product transfusion as predictors of post-traumatic stress disorder in firearm-related trauma.

Surgery·2026
Same journal

Outcomes following endovascular aortic aneurysm repair in nonagenarian patients.

Surgery·2026
Same journal

Fistulotomy with primary sphincteroplasty for complex anal fistulas: Should we be concerned about incontinence?

Surgery·2026
Same journal

No need for mesh in the repair of hiatal hernias: Autologous tissue hiatoplasty techniques for the repair of the complex diaphragmatic defect.

Surgery·2026
See all related articles

Recombinant platelet-derived growth factor (rPDGF) can stimulate tissue growth in vivo. Continuous delivery of rPDGF-BB is more effective for sustained tissue generation than pulsed delivery, but stabilization is needed for reconstructive use.

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • An in vivo experimental model was developed to assess the potential of recombinant platelet-derived growth factor (rPDGF) in generating functional tissue.
  • This research explores the use of growth factors for tissue regeneration applications.

Purpose of the Study:

  • To investigate the mitogenic effect of rPDGF in an in vivo tissue generation model.
  • To compare the efficacy of pulsed versus continuous delivery of rPDGF-BB for tissue formation.

Main Methods:

  • An arteriovenous bundle from Lewis rats was encased in silicone chambers with collagen disks releasing rPDGF-BB.
  • Tissue generation was analyzed over time, with comparisons between pulsed and sustained release methods at 30 days.
  • Histomorphometry was employed to quantify the generated tissue.

Related Experiment Videos

Main Results:

  • Pulsed rPDGF-BB delivery significantly increased tissue formation, peaking at 10-15 days, but the tissue regressed by day 30.
  • Sustained delivery of rPDGF-BB resulted in continuous tissue growth and was more effective than pulsed delivery.
  • A tenfold increase in tissue was observed with rPDGF-BB, but the tissue proved labile.

Conclusions:

  • rPDGF-BB can induce significant, albeit temporary, tissue expansion in an in vivo model.
  • Continuous delivery is crucial for maintaining the generated tissue.
  • Further strategies are required to stabilize this induced tissue for potential reconstructive applications.