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Expression and cloning of migration inhibitory factor-related protein (MRP)8 and MRP14 in arthritis-susceptible rats
T Imamichi1, I Uchida, S M Wahl
1Cellular Immunology Section, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
Abstract:
Rat migration inhibitory factor-related protein 8 (MRP8) and MRP14 were identified during screening of a subtracted cDNA library generated to identify differences in gene expression between LEW/N and F344/N rats. The predicted amino acid sequence of rat MRP8 and MRP14 is 60-80% identical with that from human and mouse. Expression of these genes correlated with chronic inflammation in LEW/N rats, but were absent in F344/N rats which do not develop arthritis in response to streptococcal cell wall peptidoglycan-polysaccharide complexes (SCW). These differences suggest a role for MRP8 and MRP14 in susceptibility to SCW-induced chronic disease.
Insights
Rat migration inhibitory factor-related protein 8 (MRP8) and MRP14 genes are linked to chronic inflammation in LEW/N rats. Their absence in F344/N rats suggests a role in susceptibility to streptococcal cell wall-induced disease.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Migration inhibitory factor-related proteins (MRPs) are involved in inflammatory processes.
- Rat strains LEW/N and F344/N exhibit differential susceptibility to streptococcal cell wall (SCW)-induced arthritis.
- Understanding genetic differences is crucial for elucidating disease mechanisms.
Purpose of the Study:
- To identify genes differentially expressed between LEW/N and F344/N rats.
- To investigate the role of MRP8 and MRP14 in the susceptibility to SCW-induced chronic disease.
Main Methods:
- Subtracted cDNA library screening was employed to identify differentially expressed genes.
- Amino acid sequence homology between rat, human, and mouse MRP8 and MRP14 was analyzed.
- Gene expression patterns were correlated with disease development in response to SCW.
Main Results:
- Rat migration inhibitory factor-related protein 8 (MRP8) and MRP14 were identified as differentially expressed genes.
- Rat MRP8 and MRP14 sequences show 60-80% identity to human and mouse orthologs.
- MRP8 and MRP14 gene expression correlated with chronic inflammation in LEW/N rats but were absent in resistant F344/N rats.
Conclusions:
- MRP8 and MRP14 expression is associated with susceptibility to SCW-induced chronic inflammatory disease in rats.
- These findings highlight a potential role for MRP8 and MRP14 in the pathogenesis of arthritis.
- Further research into MRP8 and MRP14 could offer insights into inflammatory disease mechanisms.