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Related Experiment Videos

Bacterial allergy in nasal polyposis. A new method for quantifying specific IgE

E Calenoff1, J T McMahan, G D Herzon

  • 1Department of Otolaryngology and Head and Neck Surgery, Northwestern University Medical School, Chicago, Ill. 60611-3008.

Archives of Otolaryngology--Head & Neck Surgery
|August 1, 1993
PubMed
Summary

This study found that removing IgG antibodies enhances the detection of bacteria-specific IgE in patients with chronic sinus disease. Most patients with nasal polyposis or chronic sinusitis show positive bacteria-specific IgE levels.

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Area of Science:

  • Immunology
  • Otolaryngology
  • Microbiology

Background:

  • Chronic paranasal sinus disease is a complex condition.
  • The role of specific IgE antibodies in its pathogenesis is not fully understood.
  • Current diagnostic methods may be limited by interfering antibodies.

Purpose of the Study:

  • To improve the measurement of bacteria-specific serum IgE.
  • To investigate the prevalence of bacteria-specific IgE in patients with chronic sinus disease.
  • To assess the utility of a modified radioallergosorbent test (RAST).

Main Methods:

  • A modified RAST method was used, involving absorption of serum with recProtein A to remove IgG.
  • Purified proteins from 16 bacterial species were used as allergens.

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  • Patients with nasal polyposis, chronic sinusitis, and controls with allergic rhinitis were tested.

    • Main Results:

    • Pretreatment with recProtein A significantly increased the sensitivity of bacteria-specific RAST.
    • A high prevalence of positive bacteria-specific IgE was found in patients with nasal polyposis (17/24) and chronic sinusitis (8/14).
    • Only one control subject with allergic rhinitis showed positive IgE.

    Conclusions:

    • Bacteria-specific serum IgE can be reliably quantified using this method.
    • Most patients with chronic paranasal sinus disease exhibit bacteria-specific IgE.
    • Bacterial sensitization is implicated in the immune response of chronic sinus disease.