[Detection of chromosomal numerical aberration in glioma by FISH (fluorescence in situ hybridization)]

FISH method with chromosome specific DNA probe to interphase nuclei has been useful for the analysis of chromosomal numerical aberration in human brain tumor. We applied the FISH method to 9 gliomas and one glioma cell line (KMU-100) with 4 kinds of chromosomes of number 7, 9, 10 and 17. The predominant specific aberration in glioma were revealed the increased signal numbers of chromosome 7&17 and the decreased signal numbers of chromosome 9&10. The inactivation of tumor suppressor genes and the activation of oncogenes have been suggested as the principal mechanism of tumorigenesis in human cancer. The definite oncogenes have not still been identified on chromosome 7&17 and tumor suppressor genes on chromosome 9&10. We discussed the mechanism of tumorigenesis with one or more oncogenes on chromosome 7&17 and of one or more tumor suppressor genes on chromosome 9&10 in glioma.