Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bacterial adhesion to protein-coated hydrogels

A D Cook1, R D Sagers, W G Pitt

  • 1Chemical Engineering Department, Brigham Young University, Provo, UT 84602.

Journal of Biomaterials Applications
|July 1, 1993
PubMed
Summary

Higher water content in soft contact lenses reduces bacterial adhesion, even with protein presence. This finding is crucial for preventing eye infections linked to extended wear lenses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Timing of radiotherapy (RT) after radical prostatectomy (RP): long-term outcomes in the RADICALS-RT trial (NCT00541047).

Annals of oncology : official journal of the European Society for Medical Oncology·2024
Same author

3× multiplexed detection of antibiotic resistant plasmids with single molecule sensitivity.

Lab on a chip·2020
Same author

Targeting GM-CSF for collagenase-induced osteoarthritis pain and disease in mice.

Osteoarthritis and cartilage·2020
Same author

Pulseless electrical activity following traumatic cardiac arrest: Sign of life or death?

Injury·2019
Same author

Formation of eLiposomes as a drug delivery vehicle.

Colloids and surfaces. B, Biointerfaces·2011
Same author

Swellable coatings for hearing aid applications.

Journal of biomaterials applications·2005

Area of Science:

  • Ophthalmic biomaterials
  • Microbiology
  • Polymer science

Background:

  • Extended wear soft contact lenses are associated with increased corneal bacterial infections.
  • Understanding bacterial adhesion to lens materials is critical for preventing infection.

Purpose of the Study:

  • To investigate how polymer chemistry, water content, and pre-adsorbed proteins affect Pseudomonas aeruginosa adherence to hydrogels.
  • To identify material properties that minimize bacterial adhesion on contact lenses.

Main Methods:

  • Model hydrogels simulating extended wear soft contact lens chemistries were used.
  • Hydrogels were exposed to Pseudomonas aeruginosa suspensions in a laminar flow cell.
  • Proteins (albumin, fibrinogen, desialylated fibrinogen, mucin) were pre-adsorbed onto hydrogels.

Main Results:

  • Bacterial adhesion decreased as hydrogel water content increased, irrespective of protein pre-exposure.
  • Pre-adsorbed proteins increased bacterial adhesion by approximately 45%.
  • Sialic acid residues on proteins did not significantly alter bacterial adhesion.

Conclusions:

  • Hydrogel water content is a key factor in reducing Pseudomonas aeruginosa adhesion.
  • Protein conditioning films enhance bacterial adherence to soft contact lens materials.
  • Material design focusing on higher water content may mitigate infection risk.

Related Experiment Videos