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Detection of an oestrogen receptor-like protein in human meningiomas by band shift assay using a synthetic oestrogen
S G Koehorst1, H M Jacobs, J H Thijssen
1Department of Endocrinology, Academic Hospital Utrecht, The Netherlands.
British Journal of Cancer
|August 1, 1993
Abstract:
When a ligand binding or enzyme immunoassay is used, meningiomas are found to be rich in progestin receptors (PR) whereas oestrogen receptors (ER) are virtually undetectable. A protein that can bind to an oestrogen responsive element (ERE) was detected in meningiomas, by using the band-shift assay. The binding of ER to the ERE is inhibited by the anti-ER monoclonal antibody ER-P31, which is directed to the A/B domain of the ER, indicating that the binding protein is an ER-like protein.