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V region dependent selection of persistent resting peripheral B cells in normal mice
A C Viale1, A Coutinho, R A Heyman
1Unité d'Immunobiologie, CNRS URA 359, Institut Pasteur, Paris, France.
International Immunology
|June 1, 1993
Summary
Long-lived B cells in mice show specific gene usage, with increased VHJ558 and decreased VH7183 families. This indicates V region-dependent selection drives B cell survival and persistence.
Area of Science:
- Immunology
- Molecular Biology
Background:
- B cells are crucial for adaptive immunity.
- Understanding B cell longevity and selection is key to immune memory.
Purpose of the Study:
- To compare the VH gene repertoire of long-lived splenic B cells with the general splenic B cell population.
- To investigate the role of V region selection in B cell persistence.
Main Methods:
- Studied persistent B cells using cell transfer into recipient mice.
- Investigated non-cycling resting B cells after cytostatic or antiviral treatment.
- Analyzed VH gene family usage via in situ hybridization.
Main Results:
- Persistent B cells exhibited increased VHJ558 family expression.
- A diminished representation of the VH7183 family was observed in persistent B cells.
- These findings were consistent across different methods of isolating long-lived B cells.
Conclusions:
- B cell survival and persistence in a resting state are influenced by V region-dependent selection.
- Specific VH gene families are preferentially selected for long-term B cell maintenance.
- This selection occurs in normal, non-immunized mice.