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Functional and morphological characterization of 4F7+ spleen accessory dendritic cells
M Mohamadzadeh1, H Jonuleit, G Kolde
1Department of Dermatology, Johannes Gutenberg, Universität, Mainz, Germany.
International Immunology
|June 1, 1993
Summary
The monoclonal antibody 4F7 identifies spleen dendritic cells, crucial for T cell activation. These cells are potent stimulators of T cell proliferation, aiding in immune response studies.
Area of Science:
- Immunology
- Cell Biology
Background:
- Dendritic cells (DCs) are key antigen-presenting cells in immune responses.
- A novel monoclonal antibody, mAb 4F7, has been developed to target DCs.
Purpose of the Study:
- To characterize 4F7+ spleen dendritic cells.
- To evaluate the immune function of these identified cells.
Main Methods:
- Monoclonal antibody (mAb) 4F7 production and characterization.
- Flow cytometry (FACS) and immunoperoxidase staining for cell identification.
- Mixed lymphocyte reaction (MLR) assays to assess T cell activation.
Main Results:
- mAb 4F7 identifies a distinct population of spleen dendritic cells (approximately 1% of single cell suspensions).
- These 4F7+ cells express characteristic DC markers (MHC class II, CD11c, CD45).
- 4F7+ DCs exhibit potent allogeneic T cell activation and antigen-specific T cell proliferation.
Conclusions:
- mAb 4F7 is a valuable tool for isolating and studying spleen dendritic cells.
- These cells play a significant role in initiating T cell responses.