Simultaneous demonstration of central projections of different peripheral nerves by anti-choleragenoid immunoglobulin markers
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1Department of Anatomy, Karolinska Institutet, Stockholm, Sweden.
This study demonstrates a fluorescent antibody technique where immunoglobulins (IgG) can be used as transganglionic tracers for the simultaneous demonstration of different primary afferent nerve projections to the spinal cord. Antiserum against choleragenoid (CTB) raised in goat or rabbit was mixed with CTB to obtain a solution consisting of IgG bound to CTB. The mixtures of rabbit and goat IgG bound to CTB were subsequently injected into the sciatic or femoral nerves. Following transganglionic transport, goat and rabbit IgG staining were demonstrated simultaneously in different areas of the L4 dorsal root ganglion, in the spinal cord and in the brain stem, using an immunocytochemical technique. When only goat anti-CTB antibodies or only CTB were injected into the sciatic nerve, no goat IgG immunoreactivity could be detected in the spinal cord.
This study demonstrates a fluorescent antibody technique where immunoglobulins (IgG) can be used as transganglionic tracers for the simultaneous demonstration of different primary afferent nerve projections to the spinal cord. Antiserum against choleragenoid (CTB) raised in goat or rabbit was mixed with CTB to obtain a solution consisting of IgG bound to CTB. The mixtures of rabbit and goat IgG bound to CTB were subsequently injected into the sciatic or femoral nerves. Following transganglionic transport, goat and rabbit IgG staining were demonstrated simultaneously in different areas of the L4 dorsal root ganglion, in the spinal cord and in the brain stem, using an immunocytochemical technique. When only goat anti-CTB antibodies or only CTB were injected into the sciatic nerve, no goat IgG immunoreactivity could be detected in the spinal cord.