Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hexosamine-containing macromolecules in human colon carcinomas

T Terho, M Laitio

    Scandinavian Journal of Gastroenterology
    |January 1, 1977
    PubMed
    Summary

    Colorectal carcinoma alters mucin composition, increasing non-sulphated acid mucin and hexosamine-containing substances. These changes, particularly in glycosaminoglycans and glycopeptides, are significant in transitional and carcinoma tissues compared to normal mucosa.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Diurnal and weekly rhythms of health-related variables in home recordings for two months.

    Physiology & behavior·2006
    Same author

    Concomitant monitoring of serum neuron-specific enolase and creatine kinase BB in small cell lung cancer.

    Acta oncologica (Stockholm, Sweden)·1991
    Same author

    Histogenesis of epithelial neoplasms of human gallbladder II. Classification of carcinoma on the basis of morphological features.

    Pathology, research and practice·1983
    Same author

    Histogenesis of epithelial neoplasms of human gallbladder I. Dysplasia.

    Pathology, research and practice·1983
    Same author

    Carcinoma of extrahepatic bile ducts. A histopathologic study.

    Pathology, research and practice·1983
    Same author

    Glycoconjugates of normal and metaplastic mucosa and carcinoma of the stomach.

    Pathology, research and practice·1980

    Area of Science:

    • Gastroenterology and Oncology
    • Biochemistry
    • Histopathology

    Background:

    • Colorectal cancer (CRC) is a significant global health concern.
    • Understanding biochemical changes in colorectal tissues is crucial for early detection and treatment.
    • Mucin composition and hexosamine-containing macromolecules are altered in neoplastic tissues.

    Purpose of the Study:

    • To investigate the morphological, histochemical, and biochemical differences in normal, transitional, and carcinoma areas of the colon.
    • To characterize hexosamine-containing macromolecules, including acid glycosaminoglycans and glycopeptides, in these distinct tissue regions.
    • To quantify the concentration and composition of these macromolecules in relation to colorectal carcinogenesis.

    Main Methods:

    • Morphological, histochemical, and biochemical examination of five human colon samples resected for carcinoma.
    • Isolation and characterization of hexosamine-containing macromolecules into acid glycosaminoglycans, high-molecular-weight glycopeptides, and low-molecular-weight glycopeptides.
    • Quantitative analysis of mucin types (sulphated vs. non-sulphated) and macromolecule concentrations across normal, transitional, and carcinoma tissues.

    Main Results:

    • Transitional areas showed increased non-sulphated acid mucin compared to normal mucosa, which predominantly contained sulphated mucin.
    • Total hexosamine-containing material concentration was double in carcinoma areas versus normal areas.
    • Concentrations of acid glycosaminoglycans (hyaluronate, heparan sulphate, dermatan sulphate, chondroitin sulphate) increased progressively from normal to transitional to carcinoma tissues.
    • High-molecular-weight glycopeptides had a higher degree of sulphation in normal mucosa than in cancerous tissues.
    • Low-molecular-weight glycopeptides, particularly the saline-insoluble fraction, were significantly elevated in transitional (2x) and carcinoma (4x) areas compared to normal mucosa.

    Conclusions:

    • Colorectal carcinogenesis is associated with significant alterations in mucin composition and the concentration of hexosamine-containing macromolecules.
    • The progressive increase in acid glycosaminoglycans and specific low-molecular-weight glycopeptides suggests their potential role as biomarkers in colorectal cancer.
    • These biochemical changes provide insights into the progression of colorectal neoplasia and may offer targets for future diagnostic or therapeutic strategies.

    Related Experiment Videos