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Related Experiment Videos

Virulence-associated plasmids in Rhodococcus equi

S Takai1, Y Watanabe, T Ikeda

  • 1Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.

Journal of Clinical Microbiology
|July 1, 1993
PubMed
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This study analyzed Rhodococcus equi clinical isolates, finding that virulence plasmids of 85-kb and 90-kb are common. These plasmids share DNA homology, suggesting a common origin for R. equi virulence factors.

Area of Science:

  • Veterinary Microbiology
  • Bacterial Genetics

Background:

  • Rhodococcus equi is an important pathogen causing significant disease in foals.
  • Virulence in R. equi is associated with specific plasmid-borne genes encoding secreted antigens.

Purpose of the Study:

  • To investigate the presence and characteristics of virulence plasmids in clinical isolates of Rhodococcus equi.
  • To determine the genetic relationship between different sizes of virulence plasmids found in R. equi.

Main Methods:

  • Analysis of 23 independent clinical Rhodococcus equi isolates.
  • Plasmid DNA extraction and characterization (size determination).
  • Restriction enzyme digestion and Southern blot hybridization to assess DNA homology.

Main Results:

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  • Nineteen of 23 isolates contained an 85-kb virulence plasmid; 4 contained a 90-kb plasmid.
  • All isolates expressed 15- to 17-kDa antigens and demonstrated virulence in mouse models.
  • Significant DNA homology was observed between the 85-kb and 90-kb plasmids.

Conclusions:

  • At least two distinct virulence plasmids exist in Rhodococcus equi.
  • These virulence plasmids likely share a common evolutionary origin.
  • The presence of these plasmids is strongly associated with R. equi pathogenicity.