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Related Experiment Videos

Bacteroides fragilis strains express multiple capsular polysaccharides

A Pantosti1, A O Tzianabos, B G Reinap

  • 1Bacteriology and Medical Mycology Laboratory, Istituto Superiore di Sanità, Roma, Italy.

Journal of Clinical Microbiology
|July 1, 1993
PubMed
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The capsule of Bacteroides fragilis contains two polysaccharides, PS A and PS B. This dual polysaccharide structure is common in B. fragilis strains and shows antigenic diversity, suggesting potential for serological typing.

Area of Science:

  • Microbiology
  • Immunology
  • Biochemistry

Background:

  • The capsule of Bacteroides fragilis type strain NCTC 9343 comprises two distinct polysaccharides, PS A and PS B.
  • These polysaccharides form an aggregate on the bacterial surface, producing a complex multiprecipitin profile in immunoelectrophoresis due to differing properties.

Purpose of the Study:

  • To investigate the capsular polysaccharide composition of additional Bacteroides fragilis strains.
  • To determine if the dual polysaccharide motif observed in the prototype strain is a common feature across B. fragilis species.
  • To assess the antigenic heterogeneity of B. fragilis capsular polysaccharides for potential serological applications.

Main Methods:

  • Extraction and partial purification of capsular polysaccharides from 13 B. fragilis strains.

Related Experiment Videos

  • Analysis of capsular preparations using immunoelectrophoresis at pH 7.3.
  • Reaction with homologous polyclonal antisera and specific monoclonal antibodies (to PS A and PS B) to assess antigenic profiles.

    • Main Results:

    • All tested B. fragilis strains exhibited complex precipitin profiles similar to the prototype strain.
    • Antigenic analysis revealed significant diversity among the capsular polysaccharides, with 50% showing cross-reactivity.
    • The dual polysaccharide structure appears to be a conserved feature in B. fragilis.

    Conclusions:

    • The dual polysaccharide structure of the bacterial capsule is a common characteristic of Bacteroides fragilis strains.
    • The observed antigenic heterogeneity in B. fragilis capsular polysaccharides offers a basis for developing a novel serological typing scheme.