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Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)
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Published on: April 21, 2019

Diagnostic tests in enzyme allergy

R Merget1, J Stollfuss, R Wiewrodt

  • 1Department of Pneumonology and Allergology, Krankenanstalten, Bergmannsheil, Bochum, Germany.

The Journal of Allergy and Clinical Immunology
|August 1, 1993
PubMed
Summary
This summary is machine-generated.

Skin prick tests and bronchial provocation tests are effective for diagnosing enzyme allergies in manufacturing workers. Symptoms alone do not confirm occupational allergy, highlighting the need for accurate diagnostic methods.

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Basophil Activation Test for Allergy Diagnosis
07:22

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Published on: May 31, 2021

Area of Science:

  • Occupational Health
  • Allergology
  • Pulmonary Medicine

Background:

  • Enzyme manufacturing workers frequently experience allergies.
  • Epidemiologic studies show more symptomatic individuals than detected sensitizations via skin tests or RAST.
  • This study aimed to compare diagnostic test performance against bronchial provocation tests.

Purpose of the Study:

  • To evaluate the sensitivity and specificity of skin prick tests (SPT), enzyme allergosorbent test (EAST), and immunoprint for diagnosing enzyme allergy.
  • To establish bronchial provocation tests (BPT) as the gold standard for enzyme allergy diagnosis.
  • To assess the correlation between occupational symptoms and confirmed occupational allergy.

Main Methods:

  • Conducted 82 BPTs with nine enzymes in 42 symptomatic chemical plant workers.
  • Performed SPT, EAST, and immunoprint on the same workers and 20 control subjects (10 atopic, 10 healthy).
  • Utilized specific airway conductance changes during BPT to determine positive results.

Main Results:

  • SPT demonstrated 100% sensitivity and 93% specificity at 10 mg/ml enzyme concentration.
  • EAST showed 62% sensitivity and 96% specificity.
  • BPT identified 13 workers with positive results; most showed bronchial hyperresponsiveness.

Conclusions:

  • Occupational symptoms are not always indicative of occupational allergy.
  • Bronchial provocation tests and SPT with specific enzyme extracts are reliable for diagnosing enzyme allergy.
  • SPT is a sensitive and specific tool for identifying enzyme allergy in occupational settings.