Microtubule-associated protein MAP1B expression precedes the morphological differentiation of oligodendrocytes

The microtubule-associated protein MAP1B is believed to play an important role in the outgrowth of neurites from neurons (Tucker and Matus, Dev Biol 130: 423-434, 1988). We have investigated the possibility that MAP1B might participate in the formation of processes in cultured oligodendrocytes by an analysis of the expression of MAP1B during oligodendrocyte progenitor development. The appearance of the antigens recognized by the monoclonal antibodies A2B5, O4, and O1 which define distinct stages in the maturation of progenitors, was compared with the developmental expression of MAP1B. MAP1B is first detectable in O4+ preoligodendrocytes prior to the acquisition of galactocerebroside and immediately before they develop the complex process-bearing morphology characteristic of terminally differentiated myelin-forming oligodendrocytes in the CNS. In contrast, astrocytes have negligible amounts of MAP1B. These results demonstrate that the expression of MAP1B precedes the development of the mature oligodendrocyte phenotype and suggest that interactions between microtubules and MAP1B might have a role in the formation and stabilization of myelin-forming processes.