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Related Experiment Videos

Microbiologic contamination during dental radiographic film processing

D A Stanczyk1, E D Paunovich, J C Broome

  • 1646th Medical Group/SGD, University of Texas Health Sciences Center, San Antonio.

Oral Surgery, Oral Medicine, and Oral Pathology
|July 1, 1993
PubMed
Summary

Dental radiograph processors can harbor microbial contamination, including Candida albicans and Staphylococcus aureus, even after periods of inactivity. This contamination can transfer to films during processing, leading to cross-contamination.

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Area of Science:

  • Microbiology
  • Dental Radiology
  • Infection Control

Background:

  • Automatic dental radiograph processors and daylight loaders are essential in dental practices.
  • Maintaining a sterile environment is crucial for preventing the spread of infections.

Purpose of the Study:

  • To investigate the potential for microbiologic contamination of automatic dental radiograph processors and daylight loaders.
  • To assess the persistence of contamination after simulated clinical use and inactivity periods.
  • To determine if cross-contamination occurs between dental films during the processing stage.

Main Methods:

  • Simulated clinical use with 320 vinyl intraoral radiograph packets deliberately contaminated with pure cultures of common microorganisms (Candida albicans, Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella pneumoniae).

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  • Daily culturing of processor components (inlet/outlet rollers, developer/fixer samples) and processed films.
  • Culturing of processor sites after a 72-hour inactivity period to simulate weekend downtime.
  • Processing of 24 uncontaminated control films for comparison.

    • Main Results:

    • Microbiologic contamination was confirmed in both the automatic processor and the daylight loader.
    • Contamination persisted on processor surfaces even after 48 hours of inactivity.
    • Processed dental films remained contaminated, indicating a failure of the processing to eliminate microbes.
    • Evidence of cross-contamination between films within the processor was observed.

    Conclusions:

    • Automatic dental radiograph processors and daylight loaders are susceptible to microbial contamination.
    • Standard processing may not eliminate microbial contamination from dental films.
    • Infection control protocols should address potential contamination risks associated with dental film processing equipment.