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Hemoglobin multiple emulsion as an oxygen delivery system

S Zheng1, Y Zheng, R L Beissinger

  • 1Department of Chemical Engineering, Illinois Institute of Technology, Chicago 60616.

Biochimica Et Biophysica Acta
|August 20, 1993
PubMed
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This study shows that hemoglobin (Hb) multiple emulsions can be used as a stable oxygen delivery system. These emulsions support life in rats with low hematocrit, indicating potential as a red blood cell substitute.

Area of Science:

  • Biomaterials Science
  • Biomedical Engineering
  • Drug Delivery Systems

Background:

  • Multiple emulsion technology enables in vivo delivery of sensitive biomolecules.
  • Hemoglobin (Hb) is a potential oxygen carrier but faces challenges in stability and delivery.
  • Developing stable Hb-based oxygen carriers is crucial for medical applications.

Purpose of the Study:

  • To evaluate a prototype hemoglobin (Hb) multiple emulsion as a stable oxygen delivery system.
  • To assess the feasibility of using Hb-in-oil-in-water (Hb/O/W) emulsions as a blood substitute.
  • To determine the compatibility of Hb multiple emulsions with physiological requirements.

Main Methods:

  • Encapsulation of concentrated hemoglobin (Hb) into a Hb-in-oil-in-water (Hb/O/W) multiple emulsion using mineral oil.

Related Experiment Videos

  • Evaluation of rheological properties, hydrodynamic stability, encapsulation efficiency, and met-hemoglobin generation.
  • Assessment of oxygen affinity and cooperativity compared to whole blood.
  • In vivo studies involving isovolemic exchange transfusions in rats with critically low hematocrit.

    • Main Results:

    • Hb/O/W multiple emulsions exhibited satisfactory rheological properties and hydrodynamic stability comparable to whole blood.
    • High Hb encapsulation concentration and efficiency were achieved with minimal met-hemoglobin formation.
    • The emulsions demonstrated appropriate oxygen affinity and cooperativity.
    • Isovolemic exchange transfusions successfully supported life in rats with hematocrit levels as low as 5%, with no acute toxicity observed.

    Conclusions:

    • Hb/O/W multiple emulsions possess characteristics suitable for use as a blood substitute.
    • These findings support the utility of Hb/O/W emulsions as an oxygen-carrying red blood cell substitute.
    • The technology shows promise for applications in organ perfusion media.