Structure of three acidic O-linked carbohydrate chains of porcine zona pellucida glycoproteins
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Published on: June 3, 2014
C H Hokke1, J B Damm, J P Kamerling
1Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
Abstract:
Structural analysis by 1D and 2D 1H NMR spectroscopy of three acidic O-linked oligosaccharide alditols, released from porcine zona pellucida glycoproteins by alkaline borohydride treatment, afforded the following structures: Gal beta 1-4(6SO4)GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-3GalNAc- ol Neu5Gc alpha 2-3Gal beta 1-4(6SO4-)GlcNAc beta 1-3Gal beta 1- 4GlcNAc beta 1-3Gal beta 1-3GalNAc-ol Neu5Ac alpha 2-3Gal beta 1-4(6SO4-)GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1- 3Gal beta 1-3GalNAc-ol These oligosaccharides are the smallest compounds that contain the structural elements which are present in the acidic, high-molecular mass O-linked carbohydrate chains of porcine zona pellucida glycoproteins.
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