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Related Experiment Videos

Intra-peritoneal free elastase in CAPD peritonitis

K L Donovan1, S Pacholok, J L Humes

  • 1Institute of Nephrology, Royal Infirmary, Cardiff, Wales, United Kingdom.

Kidney International
|July 1, 1993
PubMed
Summary
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Infections in continuous ambulatory peritoneal dialysis (CAPD) patients release neutrophil elastase into peritoneal fluid. This enzyme, even in its free form, may contribute to peritoneal membrane damage and inflammation during bacterial peritonitis.

Area of Science:

  • Nephrology
  • Immunology
  • Biochemistry

Background:

  • Neutrophil (PMN) recruitment is crucial for host defense in CAPD patients with bacterial peritonitis.
  • PMN degranulation during bacterial phagocytosis releases lysosomal enzymes like neutrophil elastase.
  • The role of free neutrophil elastase in CAPD peritonitis pathogenesis requires further investigation.

Purpose of the Study:

  • To quantify free and complexed neutrophil elastase in infected and normal CAPD peritoneal fluid.
  • To assess elastase activity in CAPD peritoneal fluid.
  • To correlate elastase levels with leukocyte counts and peritonitis.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) to measure neutrophil elastase complexed with alpha 1Pi (E alpha 1Pi) and free elastase.

Related Experiment Videos

  • Casein degradation assay to estimate elastase activity.
  • Comparison of levels in infected vs. normal CAPD peritoneal fluid.

    • Main Results:

    • Infected CAPD fluid showed significantly higher levels of free elastase (median 1.4 nM) and elastase activity (median 1.2 nM) compared to normal fluid (0.0 nM).
    • Elevated E alpha 1Pi complexes (median 6.2 nM) were observed in infected fluid.
    • Strong correlations were found between peritoneal leukocyte count and both immunoreactive elastase and activity.

    Conclusions:

    • Significant quantities of uninhibited neutrophil elastase are present in the peritoneal fluid of CAPD patients with acute bacterial peritonitis.
    • The presence of free elastase may play a role in peritoneal membrane damage and inflammatory responses.
    • These findings highlight potential therapeutic targets for managing peritonitis in CAPD patients.