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Updated: Aug 13, 2026

Cholesterol Efflux Assay
Published on: March 6, 2012
Cholesterol synthesis and esterification in isolated enterocytes: regulation by cholesterol and cholestyramine
J Iglesias1, D Gonzalez-Pacanowska, C Marco
1Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Granada, Spain.
Abstract:
The purpose of the present study was to investigate the physiological control of the main regulatory enzymes of cholesterol metabolism in isolated enterocytes obtained from chick duodenum, jejunum and ileum. Cholesterol feeding resulted in an inhibition of 3-hydroxy-3-methyl-glutaryl-CoA reductase and mevalonate 5-pyrophosphate decarboxylase, while cholestyramine feeding increased reductase activity in all the regions studied and decarboxylase activity only in duodenum. Cholesterol feeding markedly increased acyl-CoA:cholesterol acyltransferase, but the effects of cholestyramine were less clear. The effects on transferase activity cannot be due to differences in the availability of acyl-CoA as exogenous substrate as no significant differences were found in acyl-CoA hydrolase activity after any of the dietary treatments. The effects of cholesterol feeding were related to changes in the cholesterol content of epithelial cells, whereas in the case of cholestyramine this relationship was less apparent.
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