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Electrophoretic karyotyping of the lignin-degrading basidiomycete Phanerochaete chrysosporium
T M D'Souza1, S B Dass, A Rasooly
1Department of Microbiology, Michigan State University, East Lansing 48824-1101.
Molecular Microbiology
|May 1, 1993
Abstract:
Electrophoretic karyotyping of the two most widely studied strains of Phanerochaete chrysosporium, BKMF-1767 and ME-446, has been determined using transverse alternating field electrophoresis. The genomic DNA of BKMF-1767 was resolved into 10 chromosomes ranging in size from 1.8-5.0 Mb, amounting to a total genome size of about 29 Mb. The genomic DNA of strain ME-446, on the other hand, was resolved into 11 chromosomes, amounting to a total genome size of about 32 Mb. Lignin peroxidase genes have been localized to five chromosomes in strain BKMF-1767 and to four chromosomes in strain ME-446.