[A rapid assaying method for biotin determination and the computer program for data calculation]

A rapid auxanographic method for biotin determination was established by means of a biotin auxotrophic yeast strain, and a computer program was designed to calculate the data obtained. Its determination concentration ranges from 0.3 to 5.0 micrograms/ml. The time-tested routine work in our laboratory proved that it is rapid, precis and reliable.