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Related Experiment Videos

Differentiation between endogenous pyrogen and leukocytic endogenous mediator

C A Mapes, P Z Sobocinski

    The American Journal of Physiology
    |January 1, 1977
    PubMed
    Summary

    Endogenous pyrogen (EP) and leukocytic endogenous mediator (LEM) are distinct molecular entities. This study differentiates EP and LEM based on physical separation, activity production, and release characteristics from leukocytes.

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    Area of Science:

    • Inflammation research
    • Immunology
    • Molecular biology

    Background:

    • Polymorphonuclear leukocytes release mediators during inflammation.
    • Endogenous pyrogen (EP) causes fever; leukocytic endogenous mediator (LEM) causes metabolic changes.
    • EP and LEM were previously considered similar.

    Purpose of the Study:

    • To differentiate between endogenous pyrogen (EP) and leukocytic endogenous mediator (LEM).
    • To provide evidence for distinct molecular identities of EP and LEM.

    Main Methods:

    • Physical separation of EP from LEM.
    • Assessing mediator production by stimulated leukocytes.
    • Analyzing mediator release from granulocytes.

    Main Results:

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    • EP was physically separated from LEM.
    • LEM activity was produced without pyrogenic activity.
    • EP and LEM showed differential release from granulocytes.

    Conclusions:

    • Endogenous pyrogen (EP) and leukocytic endogenous mediator (LEM) are distinct molecular species.
    • These findings clarify the mediators of inflammatory responses.