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Colony immunoblot assay of botulinal toxin
M C Goodnough1, B Hammer, H Sugiyama
1Department of Food Microbiology and Toxicology, University of Wisconsin-Madison 53706.
Applied and Environmental Microbiology
|July 1, 1993
Abstract:
Botulinal neurotoxin in and around colonies of Clostridium botulinum types A, B, and E and of toxigenic Clostridium butyricum was detected by an enzyme-linked immunoassay procedure whereby the toxin was transferred from the agar medium to a nitrocellulose support and the immobilized toxin was probed with type-specific antibodies. The method identified the toxin types of the colonies grown from a mixed inoculum of C. botulinum serotypes. The specificity of the antitoxins for type A and B toxins was improved by adsorption of the antitoxins with the antigens of heterologous type cultures.