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Chemoenzymatic galactosialylation with integrated cofactor regeneration
P Stangier1, W Treder, J Thiem
1Institut für Organische Chemie, Universität Hamburg, Germany.
Glycoconjugate Journal
|February 1, 1993
Summary
Researchers synthesized a key trisaccharide glycoside precursor for sialylated oligosaccharides using a one-pot enzymatic reaction with cofactor regeneration. This method efficiently produces complex carbohydrates for chemical synthesis.
Area of Science:
- Carbohydrate Chemistry
- Glycoscience
- Enzymatic Synthesis
Background:
- Sialylated oligosaccharides are crucial in biological processes.
- Efficient synthesis of these complex carbohydrates is challenging.
- Specific precursors are needed for chemical synthesis of sialylated structures.
Purpose of the Study:
- To synthesize the trisaccharide glycoside Neu5Ac alpha (2-8)Gal beta (1-4)GlcNAc beta (1-O)-pent-4-ene.
- To develop a one-pot enzymatic method for this synthesis.
- To provide a precursor for chemical synthesis of sialylated oligosaccharides.
Main Methods:
- One-pot enzymatic synthesis.
- Utilized galactosyltransferase and alpha (2-6)sialyltransferase.
- Employed a complete cofactor regeneration system.
Main Results:
- Successfully synthesized the target trisaccharide glycoside.
- Demonstrated the utility of a one-pot enzymatic approach.
- Established a precursor for further chemical modifications.
Conclusions:
- The developed method provides an efficient route to a valuable sialylated oligosaccharide precursor.
- Enzymatic synthesis with cofactor regeneration is effective for complex carbohydrate preparation.
- This precursor facilitates the chemical synthesis of diverse sialylated structures.