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Chemoenzymatic galactosialylation with integrated cofactor regeneration
P Stangier1, W Treder, J Thiem
1Institut für Organische Chemie, Universität Hamburg, Germany.
Glycoconjugate Journal
|February 1, 1993
Abstract:
As a precursor for the chemical synthesis of sialylated oligosaccharides, the trisaccharide glycoside Neu5Ac alpha (2-8)Gal beta (1-4)GlcNAc beta (1-O)-pent-4-ene was synthesized starting from GlcNAc beta (1-O)-pent-4-ene, UDP-glucose and N-acetylneuraminic acid in a one pot reaction employing galactosyltransferase and alpha (2-6)sialyltransferase in a complete cofactor regeneration system.