An inwardly rectifying K+ channel in human adenomatous parathyroid cells
Related Experiment Video
Updated: Nov 7, 2026

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
Published on: September 1, 2015
A D Conigrave1, P Poronnik, P Komwatana
1Department of Physiology, University of Sydney, New South Wales, Australia.
Abstract:
The ion channel populations of cells prepared from human adenomatous parathyroid glands have been investigated using standard patch-clamp techniques with pipettes containing isotonic K(+)-rich solutions. The principal channel type observed was an inwardly rectifying K+ channel of 35 pS conductance. In addition, we have frequently observed a lower conductance (12 pS) K+ channel that appeared to conduct current in both directions. In addition to these K+ channels, we have observed non-selective cation channels and possibly CI- channels. Although we have observed occasional current transitions that might have arisen from a large conductance K+ channel similar to that observed in rat and bovine parathyroid cells, we have not been able positively to identify such a channel in human parathyroid cells, either in cell-attached or in excised inside-out patches.
More Related Videos
07:13Two Techniques to Create Hypoparathyroid Mice: Parathyroidectomy Using GFP Glands and Diphtheria-Toxin-Mediated Parathyroid Ablation
Published on: March 14, 2017
07:19Recording of Inward Rectifying K+ Currents in Freshly Isolated Basilar Artery Smooth Muscle Cells by Patch Clamp Technique
Published on: February 7, 2025
Related Concept Videos
The Parathyroid Glands
Oxyphil cells, whose functions remain elusive, emerge during late puberty, adding a layer of complexity to the parathyroid gland's intricacies. In contrast, principal parathyroid cells undertake a vital role by producing...
Reabsorption and Secretion in the DCT and Collecting Duct
The distal part of the DCT, along with the...
Synthesis and Functions of Calcitonin
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...
Skeleton and Calcium Homeostasis
Hormones and Bone Tissue
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Reabsorption and Secretion in the Loop of Henle