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Detection of gene-disruption events in Aspergillus transformants by polymerase chain reaction direct from
A Aufauvre-Brown1, C M Tang, D W Holden
1Department of Infectious Diseases and Bacteriology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Current Genetics
|July 1, 1993
Abstract:
We describe a rapid method for the identification of gene disruption events after DNA-mediated transformation of Aspergillus fumigatus. This involves a polymerase chain reaction in which the target DNA is added in the form of intact conidiospores. Using one primer specific to the plasmid DNA and a second primer specific to the target gene on the chromosome, it is possible to identify gene disruption events among the more common ectopic integrations approximately 4 h after sporulating transformants appear on selective medium.