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Updated: Aug 9, 2026
High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
An enzyme immunoassay for the direct detection of adenovirus in clinical specimens
C A Gleaves1, J Militoni, R L Ashley
1Diagnostic Virology Laboratory, Fred Hutchinson Cancer Research Center, Seattle, Washington.
Abstract:
A total of 414 clinical samples were tested by an enzyme immunoassay (EIA) for direct detection of adenovirus as well as cultured in Graham 293, A549, and human diploid foreskin fibroblast cells. Adenovirus was detected in 69 (16.7%) of 414 clinical specimens. The EIA detected adenovirus in 51 (12.3%) of 414 specimens; two were EIA positive only. Adenovirus was isolated from 67 (16.2%) of 414 specimens; 18 were culture positive only. Compared with viral isolation, EIA had a sensitivity of 73.4% and a specificity of 99.4%, with a positive predictive value of 96% and a negative predictive value of 95%.
